消毒，主要包括對假陰道和輸精管。用75％的酒精棉球對假陰道內外進行消毒，待酒精揮發完畢后用。輸精管消毒時將兩端分別浸入大于75％的酒精中，大約20分鐘后取出，讓酒精完全揮發干凈后用配好的稀釋液沖洗3次，放人已消毒的大試管中待用。
Disinfection, including the vagina and vas deferens. To disinfect the vagina inside and outside with 75% alcohol cotton ball, until the alcohol evaporates after use. The vas deferens disinfection ends respectively in greater than 75% of the alcohol, about 20 minutes, let the complete evaporation of alcohol clean after diluted with a good rinse 3 times, then put in sterilized test tube.

    稀釋液的配制。用10％的淡奶粉作為精液稀釋液，具體配制方法是用天平(質量0．10克)稱取所需的奶粉，用蒸餾水調整到所需的濃度10％，將配制好的懸浮液、紗布及若干三角瓶用蒸汽高溫消毒20分鐘，然后將懸浮液用紗布過濾到三角瓶中涂至室溫，每毫升加入青、鏈霉素各1 000單位，攪勻即可。
Dilute solution. With 10% fresh milk as semen dilution, the specific preparation method is to use the balance (mass 0.10 g) were required for milk powder, distilled water is adjusted to the required concentration of 10%, the prepared suspension, gauze and a plurality of triangular bottle steam sterilized at high temperature for 20 minutes, then the gauze filter to the river the bottle is coated to room temperature by the suspension, per ml add green, streptomycin international units 1000, mix well.
    用生理鹽水將結晶狀的“促排3號”或“2號”溶液稀釋，隨用隨配。奶棉的制作，用剪刀將醫用脫脂棉剪成2厘米見方的小方塊若干，放人大培養皿中，一起進行蒸汽高溫消毒后，倒人過濾后的淡奶粉液，涼至室溫備用。常用0．90％氯化鈉或5％葡萄糖溶液稀釋精液，稀釋倍數一般為9倍。稀釋液的溫度應與精液等溫。稀釋液注入精瓶的速度不能過快，注入后再用玻璃棒輕輕攪動，使其均勻混合，稀釋后應再作一次顯微鏡檢查，發現精子活力不強或死亡精子過多則不能用。
With the physiological saline to crystalline "Pro ranked No. 3" or "2" dilute the solution, with the use with the distribution. Milk production of cotton, using scissors to the medical absorbent cotton was cut into 2 cm square. Some people, put in Petri dishes, together with high-temperature steam sterilization, into the filtered light milk liquid, cool to room temperature standby. 0.90% sodium chloride or 5% glucose solution diluted semen dilution, generally 9 times. Dilution of semen and isothermal temperature. Speed diluent injection bottles can not be too fast, then stir gently into the glass rod, so that it is evenly mixed, dilution should be make a microscopic examination, found in the sperm vitality is not strong or dead sperm too much is not used.
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